Glasshouse evaluation of recombinant inbred lines for b. We consider fixed recombinant inbred lines rils derived either by selfing or by fullsib mating. As each ril is an inbred strain, and so can be propagated eternally, a panel of. Two recombinant inbred families have been constructed from fp populations of t232 x cm37 and c0159 tx303. Recombinant inbred lines qtl mapping using inbred strains. Tan spot ts and septoria nodorum blotch snb induced by pyrenophora triticirepentis and parastagonospora nodorum, respectively, cause significant yield losses and adversely affect grain quality.
A recombinant inbred strain or recombinant inbred line is an organism with chromosomes that incorporate an essentially permanent set of recombination events between chromosomes inherited from two or more inbred strains. Careapproved facility under specific pathogenfree conditions. Inbred lines definition of inbred lines by the free. Jun wang and colleagues report the resequencing of six elite maize inbred lines. Inbred lines article about inbred lines by the free. Pdf quantitative trait loci controlling agronomic traits in. The use of such eightway rils will require a detailed understanding of the. Development of recombinant inbred lines rils for gene discovery and deployment. Accelerating the inbreeding of multiparental recombinant. In the present study, we evaluated fruitassociated traits and conducted quantitative trait locus qtl analysis using recombinant inbred lines rils derived from a cross.
Jun 01, 2008 recombinant inbred lines derived from an advanced intercross, in which multiple generations of mating have increased the density of recombination breakpoints, are powerful tools for mapping the loci underlying complex traits. Pdf fragrance analysis using molecular and biochemical. Grow, under the appropriate assay conditions and in a random design, 4 to 20 individuals per. Founders were crossed pairwise to produce four f1 hybrids. New arabidopsis advanced intercross recombinant inbred lines. Main plots included two irrigation treatments of 70 and 140 mm evaporation. Reinventing potato as a diploid inbred linebased crop. Enter the quantitative trait data in a spreadsheet, estimate for each trait the mean and standard. Inbred the offspring of a crosspollinating plant obtained as a result of repeated forced selfpollination.
Jan 01, 20 we describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. Senthil kumari and uma ahuja and sunita jain and rajinder k. Recombinant inbred lines derived from an advanced intercross, in which multiple generations of mating have increased the density of recombination. The replication increases the precision of the results. Parent strains are crossed to create recombinants that are then inbred to isogenicity, resulting in a permanent resource for trait mapping and analysis. Comparison of introgression a and recombinant inbred. Five segregation distorted regions were found with four showing enrichment for s. Speed, 1995 modeling interference in genetic recombination. A strain is inbred when it has undergone at least 20 generations of brother x sister or offspring x parent mating, at which point at least 98. To date, the genetic basis of fruitassociated traits in c. Such populations are currently being developed in the plantbreeding community because linkage associations present in an f2 population are broken down and a population of fixed inbred lines is also created. Individual f 2 generation plants from a kb cross were selfed for five generations to produce f 5. Recombinant inbred lines rils can serve as powerful tools for genetic mapping.
Characterization of free amino acid qtls in maize opaque2 recombinant inbred lines. Recombinant inbred lines how is recombinant inbred lines. Design and construction of recombinant inbred lines. Sorghum propinquum, that segregates for many traits related to plant architecture, growth and development, reproduction, and life history.
Use of recombinant inbred lines rils to identify, locate and map. Reduce individual variation by phenotyping multiple individuals from each strain. Pandey4, xiaoping ren1, haiwen chen1, xiaomeng xue1, nian liu1, dongxin huai1, yuning chen1, xiaojing zhou1, huaiyong luo1, weigang chen1, yong lei1, kede liu2, yingjie xiao2, rajeev k. Jan 01, 2016 an inbred line is a line of genetically similar individuals that are bred with each other for uniformity. These lines were designated as ril lines kb001 to kb5.
Application of this method to a maize ril population derived from inbred lines mo17 and. Two and threelocus tests for linkage analysis using. The rootstock effect to ameliorate abiotic stress tolerance in tomato was previously tested in a population of recombinant inbred lines prils albacete et al. New arabidopsis advanced intercross recombinant inbred. Recombinant inbred lines derived from cultivars of pea for. Development of chromosomal segment substitution lines from a. Recombinant inbred lines by sibling mating 10 19 equations for sibmating 20. Donald bailey who first conceptualized their potential utility for linkage analysis while. Lines showing 90% or higher xb proportions were selected. Phenotypic data are cumulative for each inbred line and.
Recombinant inbred lines 15 table 1 abab, aaab, aabb, and abba are 1 r22, r2 equivalence classes of twolocus diplotype states in 2, 2r1 r, 1 r22, and r22, respectively. F1 and f2 generations are produced by intercrossing the inbred strains. Development of chromosomal segment substitution lines from. The lines were again increased by selfpollination of several plants within each. Development of cssl from a backcross recombinant inbred population of interspecific rice cross 17 the number of xb homozygotes, n 2 the heterozygotes, and n the total number of informative individuals. Then, 117 of the rils were selfpollinated in ohio to produce f 6.
Intermated recombinant inbred lines how is intermated. Comparison of introgression a and recombinant inbred lines b. We report the genomic structures of irils from the ibm population and illustrate how these data can be used to develop functional ge. Female control of nonrandom mating has never been genetically established, despite being linked to inbreeding depression and sexual selection. Ijms free fulltext characterization of qtls for seedling. Linkage analysis of molecular markers and quantitative trait loci in populations of inbred backcross lines of brassica napus l.
Construction of epigenetic recombinant inbred lines ep openi. Pdf to locate map genes a populatton segregating for such genes is required. We consider recombinant inbred lines obtained by crossing two given. The use of recombinant inbred lines rils for genetic. Intermated recombinant inbred lines listed as iril. Quantitative trait locus analysis of saturated fatty acids. In order to map the loci that control femalemediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line ril1 population derived from a cross between arabidopsis arabidopsis thaliana accessions vancouver van0. Genetic variation for agronomic characters and drought. Evaluation of nutritional and phytochemical variability of. Inbred strains also called inbred lines, or rarely for animals linear animals are individuals of a particular species which are nearly identical to each other in genotype due to long inbreeding.
Such a panel would be a valuable community resource. Pdf recombinant inbred lines rils are a collection of strains that can be used to map quantitative. An ril is formed by crossing two inbred strains followed by repeated selfing or sibling mating to create a new inbred line whose genome is a mosaic of the parental genomes figure 1. Columbia recombinant inbred lines rils of arabidopsis have been used in order to identify and localize chromosome regions invo. Development of chromosomal segment substitution lines. A total of 99 recombinant inbred lines rils generated from a cross between oriental azumamugi and occidental type kanto nakate gold of barley hordeum vulgare l. Analysis of natural allelic variation in arabidopsis using a. For mouse, the lxs panel of recombinant inbred strains williams et al.
In order to map the loci that control femalemediated nonrandom mating, we constructed a new advanced intercross recombinant inbred line ril1 population derived from a cross between arabidopsis arabidopsis thaliana accessions. Ril populations have been developed and used for numerous studies in many. Genomewide patterns of genetic variation among elite maize. Pdf the use of recombinant inbred lines rils for genetic. Genotyping by sequencing of 393 sorghum bicolor btx623. The authors identified over 1 million snps and 30,000 insertion or deletion polymorphisms in this agricultural crop. Recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. For the analysis of the linkage of quantitative traits, recombinant lines are useful because of their isogenic nature, because the genetic similarity of individuals allows for the replication of a quantitative trait locus analysis. First, we show that the usual estimate of recombination fraction based on ril data is biased, and we provide an estimate where the major part of that bias is removed. However, production of dura inbred line cultivars since tenera is heterozygotic and pisifera homozygotic and female sterile with oil yields superior to the best thinshelled tenera hybrid is unlikely through this approach. Thecharacteristics of the parental lines have a profound effect on those of the f 1 offspring. Mar 28, 2012 recombinant inbred lines rils are a collection of strains that can be used to map quantitative trait loci. The genomes of recombinant inbred lines complex trait.
Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean xianzhi wang guoliang jiang marci green roy a. Breeding designs for recombinant inbred advanced intercross lines. Systems genetics and systems biology analysis of paraquat. Recombinant inbred ri lines of arabidopsis thaliana arabidopsis have been generated from a cross between the ecotypes landsberg erecta and columbia. As each ril is an inbred strain, and so can be propagated eternally, a panel of rils has a number of advantages for genetic mapping. True potato seed can be easily shared among research programs, each of which is likely to collect phenotypic data on a different set of traits. Aug 20, 2004 recombinant inbred lines rils can serve as powerful tools for genetic mapping. Linkage analysis of molecular markers and quantitative trait. Detection of quantitative trait loci influencing recombination using.
Mar 15, 2011 to exploit the diversity in arabidopsis thaliana, eight founder accessions were crossed to produce six recombinant inbred line ril subpopulations, together called an arabidopsis multiparent ril ampril population. However, there are several advantages to ultimately generating inbred lines maintained as tps rather than tubers. In this study, a set of 200 recombinant inbred lines rils derived from a cross between wheat cultivars chuanmai 42 ch42 and chuanmai 55 ch55 was used to construct a highdensity genetic map and to identify quantitative trait loci qtls for stripe rust resistance using slafseq technology. Recombinant inbred line rils population development in maize genomics lab. The simplest design, random pair mating with each pair contributing. A recombinant inbred line population of tomato and its genetic map 443 derived from a cross between two inbred lines via selfbreeding and singleseed descent breeding approach until homozygosity or near homozygosity is reached 11. Tell a friend about us, add a link to this page, or visit the webmasters page for free fun content. Broman1 department of biostatistics, johns hopkins university, baltimore, maryland 21205 manuscript received august 20, 2004 accepted for publication november 5, 2004 abstract recombinant inbred lines rils can serve as powerful tools for genetic mapping. The objectives of this study were to decipher the genetics and map the resistance to ts and snb in the pbw343kenya nyangumi kn population comprising 204 f6 recombinant inbred lines rils. Distribution of parental genome blocks in recombinant inbred lines. Construction of epigenetic recombinant inbred lines ep. Inbred model organisms are powerful tools for genetic studies because they provide reproducible genomes for use in mapping and genetic manipulation. As each ril is an inbred strain, and so can be propagated eternally, a panel of rils has a number of advantages for genetic.
The authors identified over 1 million snps and 30,000 insertion or deletion polymorphisms in. We describe a genetic map with a total of 381 bins of 616 genotyping by sequencing gbsbased snp markers in a f6f8 recombinant inbred line ril population of 393 individuals derived from crossing s. Inbred lines article about inbred lines by the free dictionary. Recently, members of the complex trait consortium proposed the development of a large panel of eightway rils in the mouse, derived from eight genetically diverse parental strains. In this article we note that since recombinant inbred lines rils provide information. Additional lines were chosen from those showing 8590% xb proportions to extent the. Here i describe the process of designing and constructing rils. Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. Intermated recombinant inbred lines how is intermated recombinant inbred lines abbreviated. Broman kw 2005 the genomes of recombinant inbred lines. This is recombinant inbred line development in soybean by plant and soil sciences elibrary on vimeo, the home for high quality videos and the people. Recombinant inbred line rils population development in. Dissecting yieldassociated loci in super hybrid rice by.
Quantitative trait locus analysis using recombinant inbred. An inbred line consists of individuals with the same genotype. As a result of heterosis, hybrids from the crossbreeding of inbreds are substantially superior to. It is also implicated as a risk factor for sporadic parkinsons di. Aug 01, 2018 we describe a genetic map with a total of 381 bins of 616 genotyping by sequencing gbsbased snp markers in a f6f8 recombinant inbred line ril population of 393 individuals derived from crossing s. Although a handful of isolated recombinant inbred ri mouse strains were developed during the 1940s and 1950s, the importance of these genetic constructs was not appreciated by any scientist prior to dr. Genomewide patterns of genetic variation among elite. Generating inbred lines via sibling matings, however, is a costly undertaking that requires many successive generations of breeding, during which time many lines fail. In file s1, we provide a parameterfree approximation to. To elucidate the genetic basis of yield and uncover its associated loci in rice, we resequenced the core recombinant inbred lines of liangyoupeijiu, the widely cultivated super hybrid rice, and constructed a highresolution linkage. Genetic linkage map of a recombinant inbred lines in tomato. Recombinant inbred line development in soybean on vimeo. A induction of epigenomic perturbation by means of a mutation in genes involved in chromatin control, followed by selfing plants or sibling mating mammals of conditional intercross f2 c. Set up the appropriate quantitative assay to measure precisely the trait s.
Mccouch, s r and doerge, r w 1995 qtl mapping is rice trends genet 11,482487. Progeny of 300 individual f 2 seedlings were taken by single seed descent to the f 8 generation. Genomewide expression quantitative trait locus analysis in a recombinant inbred line population for trait dissection in peanut li huang1,2, xia liu3, manish k. Pdf characterization of free amino acid qtls in maize. It is assumed that the parental strains have nearly identical dna sequences shaded horizontal. Development of chromosomal segment substitution lines from a backcross recombinant inbred population of interspecific rice cross chen jie 1, 2, hafeez ur rahman bughio 3, chen dazhou 4, liu guangjie 1, zheng kangle 1, zhuang jieyun 1. Mapping resistance quantitative trait loci for three foliar. Recombinant inbred lines derived from cultivars of pea for understanding the genetic basis of variation in breeders traits volume 16 special issue carol moreau, maggie knox, lynda turner, tracey rayner, jane thomas, haidee philpott, steve belcher, keith fox, noel ellis, claire domoney. We describe a recombinant inbred line ril population of 161 f5 genotypes for the widest euploid cross that can be made to cultivated sorghum sorghum bicolor using conventional techniques, s. These f1s were crossed according to a diallel scheme. A multiparent recombinant inbred line population of c. The study was set up to evaluate the nutritional value, phytochemical content and antioxidant activity of cowpea recombinant inbred lines rils under contrasting soil moisture in the guinea and sudan savanna agroecologies of ghana. Paraquat pq is an herbicide used in many countries, including the united states.
On the determination of recombination rates in intermated. Genetic dissection of intermated recombinant inbred lines. The resulting offspring was then selfed for three generations. With repeated selfpollination the viability and yield of the offspring are decreased, while the homogeneity in respect to the genotype is increased. Twentyfive promising recombinant inbred tomato genotypes were selected from the 80 lines tested for indigenous whitefly b. The development of recombinant inbred lines has been proposed and attempted in oil palms. Introgression lines are created by backcrossing an f1 of a cross between two parental lines to. The use of such eightway rils will require a detailed understanding of.
The recurrent intermating of f2 individuals for some number of generations followed by several generations of inbreeding produces an intermated recombinant inbred iri population. Recombinant inbred lines by sibling mating 4 advantages of ri lines each strain is an eternal resource. Glasshouse evaluation of recombinant inbred lines for b biotype vector resistance. Genetic analysis of recombinant inbred lines for sorghum. Using crossover breakpoints in recombinant inbred lines to. Oct 24, 2010 jun wang and colleagues report the resequencing of six elite maize inbred lines. Pdf design and construction of recombinant inbred lines. Pdf correlations and path coefficient analysis in rice. Pdf on oct 3, 2019, akanksha singh and others published correlations and path coefficient analysis in rice recombinant inbred lines find, read and cite all the research you need on researchgate. We investigated the effects of intercross breeding designs on the utility of such lines for mapping. D v butruille, r p guries, and t c osborn department of agronomy, university of wisconsin, madison, wisconsin 53706, usa.
Design and construction of recombinant inbred lines article pdf available in methods in molecular biology clifton, n. The genomes of recombinant inbred lines 2 inbred mice. Fortyeight cowpea rils seed samples from wellwatered and water stress experiment were pulverized. We evaluated several approaches for accelerating inbreeding, including the.
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